Solid state fermentation ssf cultivation systems three different substrates that were paddy husks, rubber wood saw dusts and palm kernel cake were examined for the cultivation of filamentous fungi. Original article quantitative approach to track lipase. Lipase production and purification from fermentation broth using ionic liquids sonia p. Solid state fermentation ssf has been largely employed during the last three decades to produce different biomolecules of industrial interest, particularly enzymes.
The production of lipase by penicillium simplicissimumin solid state fermentation was studied using babassu cake as the basal medium. Production and optimization of lipase using aspergillus. Fungal lipase production by solid state fermentation an overview devarai santhosh kumar1 and suman ray2. Lipase production by yarrowia lipolytica using olive oil. Lipase production in ssf with dfrb using sterile water as a moisture source exhibited more activity 577. Based on results, optimum condition of submerged fermentation has been achieved with inducer concentration of 6. The aim of this work was to maximize the production of aspergillus ibericus lipase under ssf of olive pomace op and wheat bran wb, evaluating the effect on lipase production of cn ratio, lipids, phenols. Pdf lipase production in solidstate fermentation ssf. Keeping all these in view, the present work is designed for nonsterilized fermentative production of lipase by pseudomonas sp. Oil cakes as substrate for improved lipase production in.
Daily, lipase production was evaluated at several incubation temperatures. Lipase production by solidstate fermentation springerlink. Lipases have various industrial applications and their production by filamentous fungi in solid state fermentation ssf process is of interest. Among the various fungal strains screened for lipase production, rhizopus arrhizus ncim 877, 878, 879 and aspergillus niger ncim 1207 produced significant quantities of enzyme when grown in synthetic oil based sob medium under submerged conditions. Extracellular lipase production by penicillium chrysogenum, trichoderma harzianum and aspergillus flavus was carried out through solid state. Introduction lipases, also known as triacylglycerol ester hydrolases e. By solid state fermentation in wheat bran containing 2. Optimization of lipase production in solidstate fermentation by in. Screening of different agricultural wastes such as rice bran, wheat bran, canola seed oil cake, sunflower hulls and peanut shells was carried out and canola seed oil cake was selected as best substrate due to highest lipase. Lipase production by single substrate fermentation fermentation studies were investigated by the initial moisture content was 55% and inoculum concentration 2. The present study deals with the production of lipase from candida rugosa ncim 3467 using groundnut oil cake under solid state fermentation. Lipase production by aspergillus niger 11t53a14 in wheat. Solid state fermentation for the production of industrial.
Solid state fermentation of lipase p roduction from. S1 and to develop pcrbased molecular markers to trace the fate of pseudomonas sp. Lipase production by aspergillus niger in different. Bacteria among bacterial lipases being exploited, those from 10 bacillus exhibit interesting properties that make them obtained a maximum lipase activity of 1,585 u potential candidates for biotechnological applications. The chosen fungal strain was evaluated for lipase production using a mixture of agro industrial substrates viz. Bio26 selection and optimization of lipase production. Microorganism used in fermentation process was inoculated at a concentration of 10 production of lipase by submerged and solid state fermentation, procedures for obtaining the enzymatic extracts were conducted, which are described below. Lipase in solid state fermentation by yarrowia lipolytica from niger seed oil. Different oil soapstocks nd their application a n the lipase production i y. Lipases produced from microbial fermentation systems. Feb 01, 2020 in order to produce lipase in large scale, therefore the objective of this study was to optimize the production of dry a. The results obtained for two organisms were as follows. Agro wastes were collected from nearer places of bagalkot, which.
The efficiency solid state fermentation was more than. Lipase production by aspergillus niger 11t53a14 in wheat bran. Whole cell immobilization processes for lipase production have been applied directly to enhance their biocatalytic activity and to obtain prolong use. F the average specific activity of the lipase produced in ssf and slf were plotted and compared. Pdf production of acidic lipase by aspergillus niger in. Lipase production through solidstate fermentation using. However, the most significant improvement of lipase production under solid state fermentation was achieved by an alkaline treatment of the substrate more than 10fold, when. This work deals with the lipase production by ssf of olive pomace op in traditional tray. Soapstock is formed by treating refining crude or degummed vegetable oils with alkali to.
International training course on solid state fermentation horizon ird. Optimization of lipase production in solidstate fermentation. Lipase production by aspergillus niger in different agro. The aim of this work was to optimize the production of lipase by aspergillus ibericus mum 03. Submerged fermentation an overview sciencedirect topics.
Lipase production in traybioreactor via solid state. Production of lipase enzyme from rhizopus oryzae by solid state. A comparative study on alkaline lipase production by a newly. Osho, 1federal university of agriculture, college of natural sciences, department of microbiology, abeokuta, p. Kinetics of the solid state fermentation of raw cassava oour by rhizopus.
Lipase production by solid state fermentation in fixedbed bioreactors brazilian archives of biology and technology 81 fatty acids per minute under the assay conditions gombert et al. Lipase production by solidstate fermentation in fixedbed. Mudgetti re 1986 manual of industrial biotechnology. The influence factors of lipase production are the solid and liquid state and components of substrate, values of demanded water and present of agriculture residues wheat and rice bran in media. Lipase production through solidstate fermentation using agro. Through the use of agroindustrial wastes as ssf substrates, an economic process of lipases production can be achieved. Using optimized parameters, time course studies on lipase production were carried out for single substrate fermentation. A comparative study of the lipase yield by solid state and. Michael bamitale osho2 and adebukola mobolaji omemu3 addresses. Optimization of lipase production by solidstate fermentation. Recent developments and biotechnological applications ashok kumar shamsher singh kanwar department of biotechnology, himachal pradesh university, summer hill, shimla171 005, india. Particularly for lipase production, since it has residual content of olive oil. Lipases are versatile catalysts with many applications and can be produced by solid state fermentation ssf using agroindustrial wastes.
Fungal lipase production by solid state fermentation luis beltran ramossanchez 1, mario cesar cujilemaquitio, maria caridad julianricardo 1, jesus cordova 2 and patrick fickers 3,4 1 department of chemical engineering, university of camaguey ignacio agramonte loynaz circunvalacion norte, km 5. Several reports are available on lipase production by ssf using solid substrates inc luding rice bran rao et al. Production of microbial enzymes by solidstate fermentation. Lipase produced by ssf was stable over a period of 15 days, whereas lipase production in smf decreased by day 5. The optimum enzyme activity of groundnut oil cake after 96hr was found to be was 7.
The comparative study of lipase production was done to check, whether solid state fermentation ssf or submerged liquid fermentation sl gives good yield. Agricultural waste, aspergillus niger f702, lipase, optimization, solid state fermentation introduction o lipases are hydrolytic enzymes that act in aqueousorganic interfaces, catalyzing the cleavage of ester bonds in triglycerides and producing glycerol and free fatty acids freire and castilho, 2000. Solid state fermentation, lipase enzyme, pseudomonas aeruginosa i. It can be of special interest in those processes where the crude fermented product may be used directly as the enzyme source1. This was carried out to determine ph at which fungal growth is maximum. Optimization of aspergillus niger lipase production by solid. The influence of various oil cakes has been investigated for high level production of lipase using aspergillus tamarii mtcc 5152. Optimization of parameters for the production of lipase. Characterization of different oil soapstocks and their. Bio26 selection and optimization of lipase production from. Production of lipase by solid state fermentation the production was carried out in ml erlenmeyer flasks. Ramesh babu 1assistant professor, 2 professor and head dept. Current studies on lipase production by solid state fermentation involve the use of agroindustrial residues, aiming at increasing the economic attractiveness.
In this work the lipase enzyme production by the mutant aspergillus niger 11t51a14, which is a wellknown lipase producer was studied using wheat bran as substrate. It was under taken to optimize the key process variables, including incubation time, inoculum level, initial moisture content, carbon level, and ni trogen level of the medium for the production of lipase using this oil cake under ssf. Production of active lipase by rhizopus oryzae from sugarcane. Using fermentation processes for production of lipase by. Production of lipases by a newly isolate of aspergillus niger using. After the production of lipase by submerged and solid state fermentation, procedures for obtaining the enzymatic extracts were conducted, which are described below. The enzyme activity was expressed as unit of lipase activity per dried mass of fermented material ug. Sep 24, 2012 this study deals with production of lipase in solid state fermentation by rhizopus oryzae from sugarcane bagasse. Introduction lipases triacylglycerol acyl hydrolase ec 3. Solid state fermentation ssf holds tremendous poten tial for the production of enzymes. Enhanced production of aspergillus tamarii lipase for. Production and partial characterization of extracellular. Lipase production in traybioreactor via solid state fermentation under desired growth conditions 1 zahra vaseghi, ghasem d.
C and the fermentation was then conducted for 7 days under the following conditions. Production of active lipase by rhizopus oryzae from. Production of lipases by rhizopus oligosporous by solid. Dynamic biochemistry, process biotechnology and molecular biology 2012 global science books lipase production in solid state fermentation ssf.
Recent developments and biotechnological applications ashok kumar shamsher singh kanwar department of biotechnology, himachal pradesh university, summer hill, shimla171 005, india corresponding author. The present study was undertaken to examine the effectiveness of agrowastes as a substrate for lipase production, purification, and characterization of the enzyme. Production and optimization of lipase using aspergillus niger. Najafpour, samaneh mohseni,1 2,3 soleiman mahjoub and maryam n. All chemical compounds including k 2 hpo4, nacl, mgso 4. Process parameters like fermentation time, temperature, ph, inoculum age. Fermentation processes may be divided into two systems. The growing interest in lipase production is related to the potential biotechnological applications that these enzymes present.
The production of extra cellular lipase in solid state fer mentation ssf using. The authors reported also an increase in lipase activity with the addition of yeast and beef extract 17 sun sy, xu y. The optimization of the conditions and the use of low cost components in solid state fermentation makes the process economicaly effective for production of lipase from the. Production and characterization of lipases by two new. Pkc as a substrate for the production of lipase by solid state fermentation.
Solid state fermentation for the production of industrial enzymes. A comparative study on alkaline lipase production by a. In addition to the conventional applications in food and fermentation industries, microbial enzymes have attained. Recent developments and biotechnological applications find, read and. The production of lipase by penicillium simplicissimum in solid state fermentation was studied using babassu cake as the basal medium. Optimization of aspergillus niger lipase production by. Screening of different agricultural wastes such as rice bran, wheat bran, canola seed oil cake, sunflower hulls and peanut shells was carried out and canola seed oil cake was selected as best substrate due to highest lipase activity. Optimization of process parameters for the production of lipase in. The performance of solid state and submerged fermentation in producing lipase. Optimization of parameters for the production of lipase from. Solidstate fermentation as an economic production method of. Lipase productionby aspergillus niger grown in different agroindustrial wastes by solid state fermentation 421 the culture medium was sterilized in an autoclave 20 minutes at 121. All the cultures were grown in 250ml conical flasks containing 10g of substrate, 90% vw distilled water and. A tray bioreactor was designed for the extracellular enzyme production.
For lipase production under solid state fermentation, various agricultural waste substrates were used. Lipase, solidstate fermentation, rhizopus arrhizus, response surface methodology, agroindustrial wastes, biosynthesis. The fermented medium obtained under submerged fermentation by the fungi aspergillus flavus was filtered in cotton for the retention of hyphae and frozen at. Optimisation of ph initially, the lipase production was determined from culture in solid state fermentation media at 28. Lipase production was further improved by media supplementation andor change in the physical settings of the experiment. Kumar ds, ray s 2014 fungal lipase production by solid state fermentation an overview. Bioreactor type, sterilization and specific operational conditions are key factors for the scale. The results are presented and discussed in light and existing literatures. Pdf on jan 1, 2012, ashok kumar shamsher singh kanwar published lipase production in solid state fermentation ssf. In ssf the low energy input, simple design of the bioreactor. Pdf fungal lipase production by solidstate fermentation.
Hosseinpour1 1 faculty of chemical engineering, biotechnology research lab, noshirvani university of technology, babol, iran. General and microbiological aspects of solid substrate fermentation. Solid state fermentation for wholecell synthetic lipase production from rhizopus chinensis and identification of the functional enzyme. Oil cakes as substrate used for improved lipase production in solid state fermentation. Comparative study on lipase production using solid state and. Optimization of media constituents for the production of. Compared to the cost of culture medium, the solid state substrate dfrb is.
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